Effect of Grape Seed (Vitis vinifera) Extract on Growth and Blood Parameters of Ctenopharyngodon idella (Grass carp) and Carassius auratus (Goldfish)
DOI:
https://doi.org/10.64614/vzs-18Keywords:
Aquaculture, feed additives, grass carp, goldfish, grape seed extract, proanthocyanidinsAbstract
Proanthocyanidin found in the Grape Seed (Vitis vinifera) have an ameliorating effect for fish health. Ctenopharyngodon idella (grass carp) is an important specie of aquaculture having significant economic value and Carassius auratus (goldfish) is a potential aquarium fish specie. This research work was conducted to study growth and blood parameters of C. idella and C. auratus after administration of Grape Seed extract (GSE) for about 60 days.Total 150 fingerlings of C. idella and C. auratus were taken and divided into two groups, each group with 25 specimens randomly placed for unbiased results.. Experimental groups were fed with V. vinifera extract at 2.75% body weight of fish and the control group with 100% commercial basal diet. The length and weight of fishes were noted throughout the trial period keeping physicochemical parameters of water in optimum ranges. The results showed an increase in values between groups viz., survival rate (96%), increase in fork length (1.17, 3.67cm), weight gain (4.9, 11.8g), FCR (0.0227, 0.0093), SGR (8.88, 9.44), and the improvement in the count of RBCs (10.5, 7×106/µL), WBCs (46.8, 4.6×103/µL) and Hb (9.25, 6.5g/dL) for the grass carp and goldfish, respectively. Statistical analysis under CRD showed that Grape Seed extract had a significant effect on these parameters as the p-value was less than alpha (0.05%). Thus, grape seed extract (GSE) proved to be a potential growth and immunity enhancer for fish.
References
Abdulrahman, N.M., Abbas, L.M., Darweesh, D.H.A., Aref, H.A.S., Ahmed, W.M.K., Babaker, Z.M.R., & Jabar, R.S. (2013). Effect of grape seed on some blood parameters and serum components of common carp. Journal of Food Industrial Nutrition Science, 3, 169-174.
Al-Atbee, I.A., & Al-Niaeem, K.S. (2021). Efficiency of Grape (Vitis vinifera) Seed Oil on Nonspecific Immune Response and Histopathological Effects in Common Carp Cyprinus carpio Challenged with Pseudomonas aeruginosa. Biological Applied Environmental Research, 5, 130-140.
DOI: https://doi.org/10.51304/baer.2021.5.1.130
Arslan, G., Sonmez, A.Y., & Yan, K.T. (2018). Effects of grape (Vitis vinifera) seed oil supplementation on growth, survival, fatty acid profiles, antioxidant contents and blood parameters in rainbow trout (Oncorhynchus mykiss). Aquaculture Research, 49, 2256-2266.
DOI: https://doi.org/10.1111/are.13686
Blanco, A.M., Sundarrajan, L., Bertucci, J.I., & Unniappan, S. (2018). Why goldfish? Merits and challenges in employing goldfish as a model organism in comparative endocrinology research. General and Comparative Endocrinology, 257, 13-28.
DOI: https://doi.org/10.1016/j.ygcen.2017.02.001
Brown, C., Wolfenden, D., & Sneddon, L. (2018). Goldfish (Carassius auratus). In: Companion Animal Care and Welfare: UFAW Companion Animal Handbook, 2, 467-478.
DOI: https://doi.org/10.1002/9781119333708.ch23
Cudmore, B.M. & Mandrak, N.E. (2004). Biological synopsis of Grass Carp (Ctenopharyngodon idella). Canadian Manuscript Report of Fisheries and Aquatic Sciences, 2705 (7), 1-44.
Dibble, E.D. & Kovalenko, K. (2009). Ecological impact of grass carp: A review of the available data. Journal of Aquatic Plant Management, 47, 1-15.
Gao, Z., Liu, G., Hu, Z., Li, X., Yang, X., Jiang, B. & Li, X. (2014). Grape seed proanthocyanidin extract protects from cisplatin-induced nephrotoxicity by inhibiting endoplasmic reticulum stress-induced apoptosis. Molecular Medicine Reports, 9, 801-807.
DOI: https://doi.org/10.3892/mmr.2014.1883
FAO. 2022. The state of the world’s fisheries and aquaculture. Food and Agriculture Organization of the United Nations.
Garlock, T., Asche, F., Anderson, J., Bjorndal, T., Kumar, G., Lorenzen, K., & Tveteras, R. (2020). A global blue revolution: Aquaculture growth across regions, species, and countries. Reviews in Fisheries Science & Aquaculture, 28, 107-116.
DOI: https://doi.org/10.1080/23308249.2019.1678111
Gupta, M., Dey, S., Marbaniang, D., Pal, P., Ray, S., & Mazumder, B. (2020). Grape seed extract: Having a potential health benefits. Journal of Food Science and Technology, 57, 1205-1215.
DOI: https://doi.org/10.1007/s13197-019-04113-w
Hassan, H.U., Ali, Q.M., Ahmad, N., Masood, Z., Hossain, M.Y., Gabol, K., Khan, W., Hussain, M., Ali, A., Attaullah, M., & Kamal, M. (2021). Assessment of growth characteristics, survival rate and body composition of Asian sea bass (Lates calcarifer, Bloch 1790) under different feeding rates in closed aquaculture system. Saudi Journal of Biological Sciences, 28, 1324-1330.
DOI: https://doi.org/10.1016/j.sjbs.2020.11.056
Huang, G., Luo, S. & Zeng, X. (2012). Effects of plant extract from grape seed on growth and composition of muscles of hybrid crucian carp. Fisheries Science, 31, 433-436.
Jahanbakhshi, A., Pourmozaffar, S., Mozanzadeh, M.T., Adeshina, I., & Vega Heredia, S. (2023). Dietary effect of grape seed proanthocyanidin extract on growth performance, serum biochemical parameters, skin mucosal immune response, and antioxidant capacity in goldfish. Annals of Animal Science, 23, 215-223.
DOI: https://doi.org/10.2478/aoas-2022-0059
Kwatra, B. (2020). A review on potential properties and therapeutic applications of grape seed extract. World Journal of Pharmaceutical Research, 9, 2519-2540.
Laurent, C., Besançon, P., & Caporiccio, B. (2007). Flavonoids from a grape seed extract interact with digestive secretions and intestinal cells as assessed in an in vitro digestion/Caco-2 cell culture model. Food Chemistry, 100, 1704-1712.
DOI: https://doi.org/10.1016/j.foodchem.2005.10.016
Li, L., Balto, G., Xu, X., Shen, Y., & Li, J. (2023). The feeding ecology of grass carp: A review. Reviews in Aquaculture, 15(4), 1335-1354. DOI: https://doi.org/10.1111/raq.12777
Mehrinakhi, Z., Ahmadifar, E., Sheikhzadeh, N., Moghadam, M.S., & Dawood, M.A. (2021). Extract of grape seed enhances the growth performance, humoral and mucosal immunity, and resistance of common carp (Cyprinus carpio) against Aeromonas hydrophila. Annals of Animal Science, 21(1), 217-232.
Mohammadi, Y., Kamangar, B.B., & Zarei, M.A. (2021). Effects of diets containing grape seed proanthocyanidin extract on the growth and oxidative capacity of common carp (Cyprinus carpio). Aquaculture, 540, 736689.
DOI: https://doi.org/10.1016/j.aquaculture.2021.736689
Mousavi, S., Sheikhzadeh, N., Tayefi-Nasrabadi, H., Alizadeh-Salteh, S., Oushani, A.K., Firouzamandi, M., & Mardani, K. (2020). Administration of grape (Vitis vinifera) seed extract to rainbow trout (Oncorhynchus mykiss) modulates growth performance, some biochemical parameters, and antioxidant-relevant gene expression. Fish Physiology and Biochemistry, 46(3), 777-786.
DOI: https://doi.org/10.1007/s10695-019-00716-4
Nazima, B., Manoharan, V., & Miltonprabu, S. (2015). Grape seed proanthocyanidins ameliorate cadmium-induced renal injury and oxidative stress in experimental rats through the upregulation of nuclear related factor 2 and antioxidant responsive elements. Biochemistry and Cell Biology, 93, 210-226.
DOI: https://doi.org/10.1139/bcb-2020-0070
Nowshehri, J.A., Bhat, Z.A., & Shah, M.Y. (2015). Blessings in disguise: Bio-functional benefits of grape seed extracts. Food Research International, 77, 333-348.
DOI: https://doi.org/10.1016/j.foodres.2015.08.026
Parida, S.P., Dutta, S.K., & Pal, A. (2011). Hematological and plasma biochemistry in Psammophilus blanfordanus (Sauria: Agamidae). Complementary Clinical Pathology, 21, 1387-1394.
DOI: https://doi.org/10.1007/s00580-011-1303-7
Pullin, R.S. (2006). Genetic resources for aquaculture: Status and trends. In: Bartley, D.M., Harvey, B.J. & Pullin, R.S.V. (eds). Workshop on Status and Trends in Aquatic Genetic Resources: A Basis for International Policy. FAO Fisheries Proceedings, 109-143p.
Rafique, M., & Khan, N.U.H. (2012). Distribution and status of significant freshwater fishes of Pakistan. Record of the Zoological Survey of Pakistan, 21, 90-95.
Saravanan, M., Kumar, K.P., & Ramesh, M. (2011). Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: Cypriniformes) during acute and chronic sublethal exposure to lindane. Pesticide Biochemistry and Physiology, 100(3), 206-211.
DOI: https://doi.org/10.1016/j.pestbp.2011.04.002
Shiel, B.P., Hall, N.E., Cooke, I.R., Robinson, N.A., Stone, D.A., & Strugnell, J.M. (2017). The effect of commercial, natural and grape seed extract-supplemented diets on gene expression signatures and survival of greenlip abalone (Haliotis laevigata) during heat stress. Aquaculture, 479, 798-807.
DOI: https://doi.org/10.1016/j.aquaculture.2017.07.025
Stickney, R.R., & Gatlin, D.M. (2022). Aquaculture: An Introductory Text, 115-131p.
DOI: https://doi.org/10.1079/9781800621145.0000
Subasinghe, R., Soto, D., & Jia, J. (2009). Global aquaculture and its role in sustainable development. Reviews in Aquaculture, 1(1), 2-9.
DOI: https://doi.org/10.1111/j.1753-5131.2008.01002.x
Xie, L.N., Mao, T.T., Liu, C., Jiang, L.S., Guo, Y.Q., & Fang, L.Y. (2012). Effect of grape seed proanthocyanidins on enzyme activity, relative weight of intestinal organs and routine blood test for weaned piglets. Journal of Beijing University of Agriculture, 27, 17-19.
Zhai, S.W., Lu, J.J., & Chen, X.H. (2014). Effects of dietary grape seed proanthocyanidins on growth performance, some serum biochemical parameters and body composition of tilapia (Oreochromis niloticus) fingerlings. Italian Journal of Animal Science, 13(3), 3357.
DOI: https://doi.org/10.4081/ijas.2014.3357
Zhu, F. (2020). A review on the application of herbal medicines in the disease control of aquatic animals. Aquaculture, 526, 735422.
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Copyright (c) 2025 Aneela Nazir, Shakeela Parveen, Fayyaz Rasool, Arooj Haneef, Daima Nosheen, Sana Ghaffar, Muhammad Afzaal, Naila Mukhtar, Noman Sanwal, Muhammad Haroon
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