Investigation of the Probiotic Properties of Some Cattle-Origin Bacterial Isolates (Lactobacillus spp., Enterococcus faecium and Escherichia coli)
DOI:
https://doi.org/10.64614/vzs-30Keywords:
E. coli, E. faecium, Lactobacillus spp, probiotic, cowAbstract
In this study, Lactobacillus spp. (56 isolates), Enterococcus faecium (25 isolates), and Escherichia coli (33 isolates) were isolated from bovine ceca, and their probiotic properties were investigated in vitro. The isolates were evaluated for bile salt tolerance at different concentrations (0.6% and 1.5%), survival rates at pH=2 and pH=7, cell surface hydrophobicity, antibacterial activity against the E. coli K99 strain, and susceptibility to six antibiotics from five different classes (ampicillin, trimethoprim–sulfamethoxazole, neomycin, enrofloxacin, amoxicillin-clavulanic acid, and tetracycline). All E. faecium and E. coli isolates, as well as all but two Lactobacillus spp. isolates, were able to grow at 0.6% bile concentration. At 1.5% bile concentration, all E. coli isolates and all but two Lactobacillus spp. and one E. faecium isolate exhibited growth. The viability rates at pH=2 were determined as 34-52% for Lactobacillus spp. (14 isolates), 35% for E. faecium (1 isolate), and 34-55% for E. coli (4 isolates). Hydrophobicity was observed in 19 Lactobacillus spp., 14 E. faecium, and 14 E. coli isolates, respectively, while none of the isolates exhibited antibacterial activity against the E. coli K99 strain. Ampicillin was identified as the antibiotic to which the isolates showed the highest resistance, with rates of 92.85% for Lactobacillus spp., 96% for E. faecium, and 100% for E. coli. In conclusion, based on their tolerance to bile salts and low pH, hydrophobic properties, and antibiotic resistance profiles, it was revealed that especially the Lactobacillus spp. M22 isolate possesses a high potential for use as a probiotic candidate.
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