Effect of Oral Administration of Morus alba L. Powder on Gut Microbiota and Survival Indices of Hypophthalmichthys molitrix (Silver Carp) and Cirrhinus mrigala (Mori)
DOI:
https://doi.org/10.64614/vzs-33Keywords:
Morus alba L. powder, gut microbiota, survival indices, Hypophthalmichthys molitrix, Cirrhinus mrigalaAbstract
The impact of Morus alba L. powder on gut microbiota and survival indices of Silver Carp (Hypophthalmichthys molitrix) and mori (Cirrhinus mrigala) was investigated in the current study. M. alba powder has long been known to possess blood-stimulating, sedative-tonic, and restorative qualities. Sixty H. molitrix and sixty C. mrigala fish specimens were taken from the hatchery and randomly placed into groups with replicates. One group (T0) was maintained as a control and fed a basal commercial diet, while the other (T1) received a diet supplemented with 6% M. alba leaves powder based on the body weight of fish for a period of 60 days. The fish's survival rate was recorded at the end of the experimental period. To evaluate the effect on microbial communities, gut samples were collected and cultured on different media, such as Nutrient Agar (NA), Tryptic Soy Agar (TSA), and Eosin Methyl Blue (EMB). The mean colony-forming unit (CFU) of Silver Carp on NA (88.33±8.37, 25.83±3.959), TSA (261.32±14.765, 162.54±10.456), and EMB (85.43±8.567, 26.97±7.345) respectively on T0 and T1. The mean colony-forming unit (CFU) of mori on NA (425±16.876, 19.67±2.678), TSA (587.86±14.876, 128.34±6.569), and EMB (119.54±8.476, 17.45±2.567) respectively on T0 and T1. Quantitative data from different treatments were analyzed using two-factor factorial ANOVA under Complete Randomized Design (CRD). The results showed that M. alba significantly affects the bacterial count in the gut of both fish species. These findings suggest that M. alba supplementation may have the potential to influence survival rate, gastrointestinal health, and immune systems in cultured fish species.
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