Effect of Fructooligosaccharide (FOS) and Xylooligosaccharide (XOS) on growth and hematology of Hypophthalmichthys molitrix (Silver carp)
DOI:
https://doi.org/10.64614/vzs-39Keywords:
Fructooligosaccharide (FOS), growth performance, hematology, Hypophthalmichthys molitrix, Xylooligosaccharide (XOS)Abstract
This study's main goal was to assess how Fructooligosaccharide (FOS) and Xylooligosaccharide (XOS) affect the growth and hematology of Hypophthalmichthys molitrix (Silver Carp). FOS and XOS are rich in plant sugars that occur naturally in many fruits and vegetables and are also known for their digestibility, non-carcinogenic activity, lowering mortality, and increasing survival rates. For the experiment, 180 fish were collected from research ponds and allocated for sixty days in control (commercial feed) and experimental sets (FOS and XOS). A completely randomized design (CRD) was applied as the experimental design to compare treatment means. Fish in T2 with XOS gain more weight (0.685±0.082g) than T1 with FOS (0.529±0.075g) and T0 with commercial feed (0.35±0.037g). T2 had the greatest length gain (0.560±0.055cm), compared to T1 (0.505±0.049cm) and T0 (0.347±0.055cm). In comparison to T1 (1.260±0.112g) and T0 (1.156±0.11g), T2 had a greater specific growth rate (SGR) (1.364±0.161g), and compared to T1 (2.012±0.176) and T0 (2.230±0.187), T2 had a reduced feed conversion ratio (FCR) (1.787±0.154). The highest survival rate was shown in T2 (95%). T2 had higher average RBC×106/μL values (3.044±0.19) than T1 (2.628±0.184) and T0 (2.192±0.115), among other data. T2 had more WBCs×103/μL (32.68±0.279) than T1 (31.78±0.214) and T0 (30.42±0.186). The mean hemoglobin levels (g/dL) in T0, T1, and T2 were 5.106±0.029, 5.276±0.034 and 5.566±0.045, respectively. Weekly, all physicochemical parameters were monitored and maintained regularly. The findings indicated that, in comparison to FOS and commercial feed, adding XOS in fish feed proved to be more effective at enhancing H. molitrix's growth and hematology.
References
Aachary, A.A. & Prapulla, S.G. (2011).Xylooligosaccharide (XOS) as an Emerging Prebiotic: Microbial synthesis, utilization, structural characterization, bioactive properties, and applications. Comprehensive Reviews in Food Science and Food Safety, 10, 2-16. DOI: https://doi.org/10.1111/j.1541-4337.2010.00135.x
DOI : https://doi.org/10.1111/j.1541-4337.2010.00135.x
Abasubong, K.P., Li, X.F., Adjoumani, J.J.Y., Jiang, G.Z., Desouky, H.E. & Liu, W.B. (2022). Effects of dietary Xylooligosaccharide prebiotic supplementation on growth, antioxidant and intestinal immune-related genes expression in Common Carp (Cyprinus carpio) fed a high fat diet. Journal of Animal Physiology and Animal Nutrition, 106, 403-418. DOI: https://doi.org/10.1111/jpn.13669
DOI: https://doi.org/10.1111/jpn.13669
Ahmed, V.M., Abulrahman, N.M., Amin, S.A.H., Hassan, B.R. & Mohammad, S.J. (2019). Effect different level of Fructooligosaccharide (FOS) on growth parameters and feed utilization in Common Carp (Cyprinus carpio). Journal of Animal and Poultry Production, 10, 285-287. DOI: https://doi.org/10.21608/jappmu.2019.54805
DOI: https://doi.org/10.21608/jappmu.2019.54805
Eissa, E.S.H., Ahmed, R.A., Aziz, Y.M.A., Hendam, B.M., Eissa, M.E. & Banna, N.I. (2024). Impact of varied combinatorial mixture of β-1, 3 Glucan and Fructooligosaccharides on growth performance, metabolism, intestinal morphometry, expression of antioxidant-related genes, immunity and protection against Vibrio alginolyticus in Red Tilapia (Oreochromis niloticus × O. mossambicus). Aquaculture International, 32, 6575-6595. DOI: https://doi.org/10.1007/s10499-024-01479-3
DOI: https://doi.org/10.1007/s10499-024-01479-3
FAO. (2020). The state of the world’s fisheries and aquaculture. Food and Agriculture Organization of the United Nations.
Fatima, A., Parveen, S., Rasool, F., Arooj, B., Shahid, Z.F., Mustafa, I., Rashid, B., Orba, F.T. & Batool, F. (2026). Effect of soybean meal (SBM) on growth and hematological parameters of rohu (Labeo rohita) and grass carp (Ctenopharyngodon idella). International Journal of Applied and Experimental Biology, 1(1). DOI: https://doi.org/10.56612/ijaaeb.v1i1.189
DOI: https://doi.org/10.56612/ijaaeb.v1i1.189
Fazio, F. (2019). Fish hematology analysis as an important tool of aquaculture: A review. Aquaculture. 500, 237-242. DOI: https://doi.org/10.1016/j.aquaculture.2018.10.030
DOI: https://doi.org/10.1016/j.aquaculture.2018.10.030
Harikrishnan, R.J., Devi, G., Doan, H.V., Tapingkae, W., Balasundaram, C., Arockiaranj, J. & Ringo, E. (2022). Changes in immune genes expression, immune response, digestive enzymes-antioxidant status, and growth of Catla (Catla catla) fed with Astragalus polysaccharides against edwardsiellosis disease. Fish and Shellfish Immunology, 121, 418-436. DOI: https://doi.org/10.1016/j.fsi.2022.01.022
DOI: https://doi.org/10.1016/j.fsi.2022.01.022
Haroon, M., Parveen, S., Rasool, F., Arooj, B., Habib, S., Godya, Z., Mukhtar, N., Ashfaq, A. & Anees, A. (2026). Changes in gut microbiota, hematological parameters, and immune response following dietary administration of oyster mushroom (Pleurotus ostreatus) in silver carp (Hypophthalmichthys molitrix). International Journal of Applied and Experimental Biology, 1(1). DOI: https://doi.org/10.56612/ijaaeb.v1i1.190
DOI: https://doi.org/10.56612/ijaaeb.v1i1.190
Hoseinifar, S.H., Soleimani, N. & Ringo, E. (2014). Effects of dietary Fructooligosaccharide supplementation on the growth performance, hemato-immunological parameters, gut micro-biota and stress resistance of Common Carp (Cyprinus carpio) fry. British Journal of Nutrition, 112, 1296-1302. DOI: https://doi.org/10.1017/S0007114514002037
DOI: https://doi.org/10.1017/S0007114514002037
Jawdhari, A., Mihailescu, D.F., Fendrihan, S., Jujea, V., Linu, V.S. & Negrea, B.M. (2022). Silver Carp (Hypophthalmichthys molitrix) presence in Danube delta and Romania-a review with data on natural production. Life, 12, 1582. DOI: https://doi.org/10.3390/life12101582
DOI: https://doi.org/10.3390/life12101582
Jin, Q., Feng, C., Xia, P., & Bai, Y. (2022). Hardness-dependent water quality criteria for protection of freshwater aquatic organisms for silver in China. International journal of environmental research and public health, 19(10), 6067. DOI: https://doi.org/10.3390/ijerph19106067
DOI: https://doi.org/10.3390/ijerph19106067
Khan, M.W. & Abbas, G. (2023). Studies on the use of aquatic food in Pakistan. Journal of Zoology and Systematics, 1, 40-57. DOI: https://doi.org/10.56946/jzs.v1i2.246
DOI: https://doi.org/10.56946/jzs.v1i2.246
Khizar, A., Fatima, M., Khan, N. & Rashid, M. (2024). Xylooligosaccharide supplementation in rice protein concentrate based diets: A comprehensive analysis of performance and health of Labeo rohita. Journal of Animal Physiology and Animal Nutrition, 108, 1059-1071. DOI: https://doi.org/10.1111/jpn.13951
DOI: https://doi.org/10.1111/jpn.13951
Lugert, V., Thaller, G., Tetens, J., Schulz, C. & Krieter, J. (2016). A review on fish growth calculation: multiple functions in fish production and their specific application. Reviews in Aquaculture, 8, 30-42. DOI: https://doi.org/10.1111/raq.12071
DOI: https://doi.org/10.1111/raq.12071
Maan, O., Parveen, S., Rasool, F., Haneef, A., Ameen, A., Haroon, M. & Mukhtar, N. (2025). Influence of Baker’s Yeast and Aspergillus oryzae on growth and microbial composition of Silver Carp (Hypophthalmichthys molitrix). Veterinary and Zootechnical Sciences, 1(2), 131–144. DOI: https://doi.org/10.64614/vzs-16
DOI: https://doi.org/10.64614/vzs-16
Malik, S., Hussain, S. & Waqas, M.S. (2020). Effect of water quality and different meals on growth of Catla catla and Labeo rohita. Big Data in Water Resource Engineering, 1, 1-5. DOI: https://doi.org/10.26480/bdwre.01.2020.04.08
DOI: https://doi.org/10.26480/bdwre.01.2020.01.05
Memon, N. A., & Kasbit, D. (2016). Fish consumption in Pakistan lowest in the world. Korea, 25, 18-321.
Morshedi, V., N. Agh, F. Noori, F. Jafari, A. Tukmechi, Marammazi, J.G. & Pagheh, E. (2018). Effects of dietary Xylooligosaccharide on growth and feeding performance, body composition and physiological responses of Sobaity Seabream (Sparidentex hasta) juvenile. Aquaculture Nutrition, 24, 1796-1803. DOI: https://doi.org/10.1111/anu.12818
DOI: https://doi.org/10.1111/anu.12818
Naylor, R., Fang, S. & Fanzo, J. (2023). A global view of aquaculture policy. Food Policy, 116, 102-422. DOI: https://doi.org/10.1016/j.foodpol.2023.102422
DOI: https://doi.org/10.1016/j.foodpol.2023.102422
Nazir, A., Parveen, S., Rasool, F., Haneef, A., Nosheen, D., Ghaffar, S. & Haroon, M. (2025). Effect of Grape Seed (Vitis vinifera) Extract on growth and blood parameters of Ctenopharyngodon idella (Grass carp) and Carassius auratus (Goldfish). Veterinary and Zootechnical Sciences, 1(2), 172–184. DOI: https://doi.org/10.64614/vzs-18
DOI: https://doi.org/10.64614/vzs-18
Prakoso, Y.A., Babazadeh, D. & Wijayanti, A.D. (2023). Potency of Desert Rose (Adenium obesum (Forssk.) Roem. and Schult.) Flower extract against artificially induced Furunculosis in Oranda goldfish (Carassius auratus auratus). Pakistan Veterinary Journal, 43, 339-344. DOI: https://doi.org/10.29261/pakvetj/2023.024
DOI: https://doi.org/10.29261/pakvetj/2023.024
Saravanan, M., Prabhu, K.K. & Ramesh, M. (2011). Hematological and biochemical responses of freshwater Teleost fish (Cyprinus carpio) during acute and chronic sub-lethal exposure to lindane. Pesticide Biochemistry and Physiology, 100, 206-211. DOI: https://doi.org/10.1016/j.pestbp.2011.04.002
DOI: https://doi.org/10.1016/j.pestbp.2011.04.002
Shimoda, K., Hamada, H. & Hamada, H. (2011). Synthesis of Xylooligosaccharides of daidzein and their anti-oxidant and anti-allergic activities. International Journal of Molecular Sciences, 12, 5616 - 5625. DOI: https://doi.org/10.3390/ijms12095616
DOI: https://doi.org/10.3390/ijms12095616
Tacon, A.G. (2022). Trends in global aquaculture and aqua-feed production: 2000-2017. Reviews in Fisheries Science and Aquaculture, 1, 43-56. DOI: https://doi.org/10.1080/23308249.2019.1649634
DOI: https://doi.org/10.1080/23308249.2019.1649634
Verdegem, M., Buschmann, A.H., Latt, U.W., Dalsgaard, A.J. & Lovatelli, A. (2023). The contribution of aquaculture systems to global aquaculture production. Journal of the World Aquaculture Society, 2, 206-250. DOI: https://doi.org/10.1111/jwas.12963
DOI: https://doi.org/10.1007/s12571-021-01246-9
Witeska, M., Kondera, E., Lugoswska, K. & Bojarski, B. (2022). Hematological methods in fish-not only for beginners. Aquaculture, 547, 737498. DOI: https://doi.org/10.1016/j.aquaculture.2021.737498
DOI: https://doi.org/10.1016/j.aquaculture.2021.737498
Zhang, Z.H., Chen, M., Xie, S.W., Chen, X., Liu, Y., Tian, L. & Niu, J. (2020). Effects of dietary Xylooligosaccharide on growth performance, enzyme activity and immunity of juvenile Grass Carp, Ctenopharyngodon idellus. Aquaculture Reports, 18, 100519. DOI: https://doi.org/10.1016/j.aqrep.2020.100519
DOI: https://doi.org/10.1016/j.aqrep.2020.100519
Zhang, C., Jiang, D., Shi, H., Zhang, C., Yang, F., Qi, Q. & Xu, R. (2024). Protective effect of Fructooligosaccharide against oxidative stress and apoptosis induced by Aeromonas hydrophila in Megalobrama amblycephala. BMC Genomics, 25, 975. DOI: https://doi.org/10.1186/s12864-024-10881-3
DOI: https://doi.org/10.1186/s12864-024-10881-3
Zhou, D., Zhang, T., Ren, L., Fang, D.A. & Xu, D.P. (2022). Differential study of microbiota in the gill and intestine of Silver Carp (Hypophthalmichthys molitrix) from the algae-dominated and hydrophyte-dominated areas of Taihu lake, China. Fishes, 7, 304. DOI: https://doi.org/10.3390/fishes7060304
DOI: https://doi.org/10.3390/fishes7060304
Zu, B., Li, W., Yang, Q., Guo, J., An, J., Li, J. & Mei, X. (2023). Ingestion of microplastics by Silver Carp (Hypophthalmichthys molitrix) larvae: Quantification of ingestion and assessment of microbiota dysbiosis. Aquatic Toxicology, 257, 106475. DOI: https://doi.org/10.1016/j.aquatox.2023.106475
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